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KMID : 1134820190480050524
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Journal of the Korean Society of Food Science and Nutrition
2019 Volume.48 No. 5 p.524 ~ p.532
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Cardioprotective Effects of Nobiletin and Tangeretin on Ischemia/Reperfusion-Induced Myocardial Injury in Rats
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Lim Sun-Ha
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Abstract
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Myocardial infarction is a heart injury caused by prolonged ischemia by the occlusion of the coronary arteries. This study evaluated effects of nobiletin (NOB) and tangeretin (TAN), which are the active compounds in an ethanol extract of Aurantii Immaturi Pericarpium, on the improved survival of myocardial cells by inhibiting apoptosis. A 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay for cell viability and western blotting for cleaved PARP and caspase-3 showed that NOB and TAN improved the viability of rat cardiomyoblasts (H9c2) by inhibiting apoptosis under hypoxic conditions. An intraperitoneal injection of NOB (1 mg/kg) or TAN (1 mg/kg) to rats prior to occlusion significantly attenuated the heart injury in a rat model of ischemia-reperfusion generated by a ligation of the left anterior descending coronary artery followed by the release of ligation. Terminal deoxynucleotidyl transferase dUTP nick-end labeling staining was performed to examine whether these effects occur by inhibiting apoptosis. In the NOB or TAN-treated groups, the number of apoptotic cells was reduced significantly in the border zone area of the heart, compared to the control group. The NOB or TAN-treatment inhibited apoptosis by modulating the biomarkers involved in the apoptotic cascade: increase in Bcl-2/Bax ratio, inhibition in the conversion of procaspase-3 to cleaved caspase-3, and decrease in the generation of DNA nicks. Overall, the intake of NOB and TAN can be beneficial for preventing myocardial infarction.
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KEYWORD
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nobiletin, tangeretin, myocardial injury, apoptosis, Aurantii Immaturi Pericarpium
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